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Which characters cannot be used in file names in MassLynx? - WKB2435

Article number: 2435


  • Windows
  • MassLynx
  • TargetLynx
  • FractionLynx
  • MaxEnt
  • DynamX 3.0
  • BioPharmaLynx


General restrictions imposed by Windows OS

  1. The names of all files are restricted to 259 characters by the operating system/disk formatting system. For normal Windows use with a standard NTFS format drive, the file name and the file path should be less than 259 characters long in total. See
  2. The OS reserves the following characters and they should not be used in file names:

< (less than)
> (greater than)
: (colon)
" (double quote)
/ (forward slash)
\ (backslash)
| (vertical bar or pipe)
? (question mark)
* (asterisk)

3.    The only characters recommended for MassLynx file naming use are:

Additional MassLynx restrictions

  1. Do not use a decimal point / full stop / period ' . ' in a file name. This can cause files to become corrupted (particularly inlet files)
  2. Do not use spaces in file paths and names. Use an underscore instead, for example: C:\Users\waters\Documents\My_data_files\File_Name.raw, instead of C:\Users\waters\Documents\My data files\File Name.raw
  3. There is a 40 character length limitation on the names of MassLynx MS methods (.exp files)
  4. There is a 55 character length limit for function names in MS Methods
  5. TargetLynx restricts compound names to 80 characters or less.
  6. Maxent 1 has also been reported to crash when file names with more than 40 characters are used.
  7. Do not use square brackets or colons in DynamX 3.0 file names. Exporting plots from DynamX 3.0 has been reported to fail if these characters are present, for example: 001 Sample:HC[2-452] EVQLVESGGGLVQPGR.
  8. NeoLynx will not process files if they contain spaces. Ensure that there are no spaces accidentally added at the end of file names.
  9. Avoid Greek characters. For example, problems occur with mu when used to represent micro (µg). This can even cause problems for some of our stand-alone data analysis applications when only used in the sample description column of the MassLynx sample list as the sample description.




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