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Comparing UV Spectra from Different Channels in a Report, at Various Retention Times - WKB120616

Article number: 120616

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Tip #204: Comparing UV Spectra from Different Channels in a Report, at Various Retention Times

The previous tip (Tip #203) discussed comparing spectra from different 3D PDA channels at the same retention time.

This tip describes how to report spectra from different 3D PDA channels that are extracted at various retention times. This is achieved by building a library of UV spectra, and then reporting on the library.

ENVIRONMENT

  • Empower

PROCEDURE

  1. Right click on the ‘Channels’ of data and select ‘Review’.

    Empower ToW #144 Figure_1.png
     
  2. Extract a chromatogram and either manually integrate the peaks of interest, or use a processing method. From the ‘Spectrum Review’ menu, select ‘Keep Spectra when Switching Channels’.

    Empower ToW #144 Figure_2.png
     
  3. Go to the next ‘3D channel’, extract a chromatogram, and integrate the peaks.

    Empower ToW #144 Figure_3.png
     
  4. From the ‘Library’ menu, select ‘New Library’. Enter a name for the library and click ‘Create’.

    Empower ToW #144 Figure_4.png
     
  5. To add the spectra to the library, go to the ‘Library’ menu, and select ‘Add to library <library name>’.

    Empower ToW #144 Figure_5.png
     
  6. In the ‘Add Spectrum to Library’ dialogue, enter a name and description and click ‘OK’. Repeat for all spectra and click ‘OK’.

    Empower ToW #144 Figure_6.png


    Now the library is created, build the Report Method
     
  7. Go to the ‘Methods’ tab, right click, and select ‘New Method’ > ‘Reporting Method’.

    Empower ToW #144 Figure_7.png
     
  8. When Report Publisher opens, click 'Cancel' to dismiss the ‘New Method/Group’ dialogue.

    Empower ToW #144 Figure_8.png
     
  9. Expand ‘PDA/MS Groups’, then expand ‘Spectral/Library Plots’, and double click on ‘Library Plot Group’.

    Empower ToW #144 Figure_9.png
     
  10. Open ‘Configuration Manager’, select ‘Libraries’, and size the window so it is on top of the ‘Report Publisher’ window. Click and drag the library from ‘Configuration Manager’ into the ‘Report Publisher’.

    Empower ToW #144 Figure_10.png
     
  11. When the ‘Open Report Method’ dialogue opens, select the last option and click ‘OK’.

    Empower ToW #144 Figure_11.png
     
  12. Close Preview, then right click on the spectral plot, and select ‘Spectral Plot Properties’.

    Empower ToW #144 Figure_12.png
     
  13. On the ‘Spectra’ tab, adjust the UV labels.

    Empower ToW #144 Figure_13.png
     
  14. On the ‘Legend’ tab, expand UV Spectra, and double click ‘Name’.

    Empower ToW #144 Figure_14.png

    An overlay of the spectra with a legend under the plot is displayed.

    Empower ToW #144 Figure_15.png
     

ADDITIONAL INFORMATION

You can use either the Pro or QuickStart interface for this tip.

EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP

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