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Comparing UV Spectra from Different Channels in a Report, at the Same Retention Time - WKB120428

Article number: 120428

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Tip #203: Comparing UV Spectra from Different Channels in a Report, at the Same Retention Time

The previous tip (Tip #202) discussed using the eCord™ with ACQUITY and ACQUITY Arc systems to automate column management and support a control strategy for column usage.

This tip discusses the overlay of UV spectra.

ENVIRONMENT

  • Empower

PROCEDURE

  1. Highlight the 3D channels, right-click and select Preview/Publisher.

    210108_1.png
     
  2. When the Open Report Method dialog window opens, select "Use the currently opened Report Method named Untitled" and click OK.

    210108_2.png
     
  3. Right-click in the empty space on the right-hand side of the window and select Method Properties. 

    210108_3.png
     
  4. Under Report Type, select Summary By All and click OK.

    210108_4.png
     
  5. On the left-hand side of the window, expand PDA/MS groups, expand Extracted Spectral Plots, and double-click on the "254nm Extraction Group".

    210108_5.png
     
  6. Right-click on the group and select Extraction Plot Properties.

    210108_6.png
     
  7. On the Chromatogram tab, delete line 1.

    210108_7.png
     
  8. On the Spectra tab, make the changes as shown in the image below and enter the appropriate extraction time for the spectrum.

    210108_8.png
     
  9. On the Overlay tab, select "Make Stack Plot" or "Overlay in Single Plot".

    210108_9.png
     
  10. On the Legend tab, delete the Plot Title entry.

    210108_10.png
     
  11. Expand "Sample".

    210108_11.png
     
  12. Double-click "SampleName".

    210108_12.png
     
  13. You now have an overlay of spectra from two different 3D PDA channels at the same retention time.

    210108_13.png

ADDITIONAL INFORMATION

Final note: You can use either the Pro or QuickStart interface for this tip.

EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP

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