Comparing UV Spectra from Different Channels in a Report, at the Same Retention Time - Tip203

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Tip #203: Comparing UV Spectra from Different Channels in a Report, at the Same Retention Time

The previous tip (Tip #202) discussed using the eCord™ with ACQUITY and ACQUITY Arc systems to automate column management and support a control strategy for column usage.

This tip discusses the overlay of UV spectra.

ENVIRONMENT

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PROCEDURE

1. Highlight the 3D channels, right-click and select Preview/Publisher.
   
   
    
2. When the Open Report Method dialog window opens, select "Use the currently opened Report Method named Untitled" and click OK.
   
   
    
3. Right-click in the empty space on the right-hand side of the window and select Method Properties. 
   
   
    
4. Under Report Type, select Summary By All and click OK.
   
   
    
5. On the left-hand side of the window, expand PDA/MS groups, expand Extracted Spectral Plots, and double-click on the "254nm Extraction Group".
   
   
    
6. Right-click on the group and select Extraction Plot Properties.
   
   
    
7. On the Chromatogram tab, delete line 1.
   
   
    
8. On the Spectra tab, make the changes as shown in the image below and enter the appropriate extraction time for the spectrum.
   
   
    
9. On the Overlay tab, select "Make Stack Plot" or "Overlay in Single Plot".
   
   
    
10. On the Legend tab, delete the Plot Title entry.
    
    
     
11. Expand "Sample".
    
    
     
12. Double-click "SampleName".
    
    
     
13. You now have an overlay of spectra from two different 3D PDA channels at the same retention time.
    
    

ADDITIONAL INFORMATION

Final note: You can use either the Pro or QuickStart interface for this tip.