How is the Dilution field used in Empower? - WKB3835

ENVIRONMENT

• Empower
• Empower 2
• Empower 3

ANSWER

Use the "Dilution" field, located in the Sample Set, when the quantity of the analyte within the sample is higher than the quantity you want to inject, yet you want to report the quantity of analyte in the original, undiluted solution. If, for instance, you make a 1:10 dilution of the solution before transferring it into the vial, enter the value "10" in the dilution field for that sample in the Sample Set. Empower then uses this value when calibrating standard injections or quantitating unknown sample injections.

For standards, Empower takes the amount or concentration of the standard and divides it by the Dilution value. It then uses the result as the x coordinate on the calibration curve.

For unknowns, Empower reads the x value directly from the calibration curve, and then multiplies that value by the Dilution value to generate a final result.

Keep in mind that Empower always uses the Dilution field value for both calibrating and quantitating. This is the reason for the default value of "1" in a new Sample Set.

ADDITIONAL INFORMATION