Cannot inject higher than 20-ul injection volume with Agilent 1290, G4226A, and Empower - WKB2946
Article number: 2946
SYMPTOMS
- Cannot inject higher than 20-ul injection volume after firmware upgrade from A.06.53 to A.06.54
- No changes in method
- Getting error "method download failed" when running samples in Empower
ENVIRONMENT
- Agilent 1290
- Empower 3
- Agilent ICF 2.1 and above
CAUSE
Seat capillary setting incorrect.
FIX or WORKAROUND
- Install the extended seat capillary, which is attached to the loop already installed. (Autosampler is supplied with a default 20-ul loop.)
- Connect the handheld controller. Go to Configure > Sampler > Syringe Volumes and change the seat capillary to the value of the installed extended seat capillary.
-
In the Empower instrument method, go to the Instrument Configuration tab.
-
Highlight Sampler, and then click configure.
-
In the Configure Sampler window, in Options, change the syringe size.
See How to determine the Agilent 1100 maximum injection volume in Empower for a screenshot.
ADDITIONAL INFORMATION
Click on the following link from Agilent for further information about the volume kit mentioned and how to check the volume.
Agilent 1290 Infinity autosampler
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