Retention time differs between a single wavelength and an extracted single wavelength from the same injection - WKB10308
Article number: 10308
SYMPTOMS
- A 3D channel with an extracted wavelength and a 2D channel of the same wavelength have different retention times for their peaks
ENVIRONMENT
- Xcalibur
- ACQUITY UPLC PDA eLambda detector
CAUSE
The 3D scan and the 2D channel use different resolution values in the instrument method.
FIX or WORKAROUND
- Change the resolution values so they match.
- Rerun.
ADDITIONAL INFORMATION
Example chromatogram
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