Empower 3: The Amount for Unknown is blank; Processing code Q08 appears - WKB100179

SYMPTOMS

• The Amount for Unknown is blank; Processing code Q08 appears

ENVIRONMENT

• Empower 3

CAUSE

Q08: Problem calculating amount or concentration, negative value calculated.
The peak area value or height value is low and it is in the negative region of the calibration curve.

FIX or WORKAROUND

1. Prevent the y-intercept of the calibration curve from increasing by running a standard with a lower concentration and adding the minimum point of the calibration curve.
2. Change the fit type of the calibration curve to the "linear through zero" (the calculated quantitative value may differ from the actual value, so it is necessary to consider when selecting this method)

ADDITIONAL INFORMATION