How does salt concentration in the matrix affect the derivatization process in AccQ-Tag Ultra? - WKB87212

ENVIRONMENT

• AccQ-Tag Ultra
• Amino Acid Analysis (AAA)
• Sodium Chloride
• Salt
• Matrix
• Derivatization 

ANSWER

Salt in the matrix, such as Sodium Chloride (NaCl), produces HCl, which lowers the pH. The pH of the sample must be between 8.2 to 10 for the derivatization reagent to react with unprotonated amines. 

ADDITIONAL INFORMATION

You must compensate for the extra salt content in the matrix. You can raise the pH by adding the same molar ratio as the salt of sodium hydroxide in the borate buffer. In addition, if the salt content is too high, precipitation of insoluble acidic salt may occur. Waters recommends dilution or scaling up to aid with this. The derivatization reagent should still be 20% of total volume.

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