Skip to main content
Waters

Can I increase my sample volume to 20 µL using AccQ•Tag Ultra on an H-Class? - WKB53980

Article number: 53980

ENVIRONMENT

  • AccQ•Tag Ultra
  • H-Class

ANSWER

The key is to keep the borate buffer at about 80% of the total composition of the mixture (borate buffer/sample/derivatization reagent). Otherwise, peak shape could be affected, especially on the early-eluting peaks. Also, it is important that the pH stays within the working range of the method.

ADDITIONAL INFORMATION

 

id53980, 2695UP

Not able to find a solution? Click here to request help.