Can I increase my sample volume to 20 µL using AccQ•Tag Ultra on an H-Class? - WKB53980
Article number: 53980
ENVIRONMENT
- AccQ•Tag Ultra
- H-Class
ANSWER
The key is to keep the borate buffer at about 80% of the total composition of the mixture (borate buffer/sample/derivatization reagent). Otherwise, peak shape could be affected, especially on the early-eluting peaks. Also, it is important that the pH stays within the working range of the method.
ADDITIONAL INFORMATION
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