How can I determine what percent organic my compound would be when it came off a reverse phase column? - WKB54082
Article number: 54082
ENVIRONMENT
Column
ANSWER
Run a very slow gradient starting with a low concentration of organic (3-5%) running to a high concentration of organic (95- 100%). Once this concentration is noted, you can develop your method around this concentration either with isocratic conditions or by using a focused gradient.
ADDITIONAL INFORMATION
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