Is adding acid to precipitate a protein the same as hydrolyzing the protein? - WKB31873
Article number: 31873
ENVIRONMENT
- Sample preparation
- Solid-phase extraction (SPE)
- Oasis
- Sep-Pak
ANSWER
No. The addition of strong acid to a protein sample will denature the tertiary structure of the protein, but this is not hydrolysis. Hydrolysis breaks down a protein into the individual amino acids.
ADDITIONAL INFORMATION
Hydrolysis of a protein involves the acid with the addition of heat and time.
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