Can I use methanol for plasma samples the Ostro Protein Precipitation and Phospholipid Removal Plate, 25 mg, 1/pkg (186005518)? - WKB32046
Article number: 32046
ENVIRONMENT
- Ostro
- Sample Preparation
ANSWER
No. Use acetonitrile as the protein precipitation solvent.
ADDITIONAL INFORMATION
Using methanol as a protein precipitation solvent for plasma samples on Ostro plates results in the formation of a gelatinous material, as opposed to a solid precipitate. This causes poor flow through of samples on Ostro plates. Waters recommends using acetonitrile because the proteins will form a solid precipitate and allow for better flow through of the supernatant on the Ostro plate.
For additional information see Ostro Sample Preparation Products Care and Use Manual
id32046,