What is the packed column volume for the ACQUITY BEH450 SEC column? - WKB88312
Article number: 88312
ENVIRONMENT
- ACQUITY BEH450 SEC
ANSWER
Vo = exclusion volume: Use approximately 0.4 x empty SEC/GPC column volume
and
Vt = total retention volume (words used in Empower GPC software parameter) [totally included volume]: Use approximately 0.8 x empty SEC/GPC column volume
ADDITIONAL INFORMATION
Calibration of actual molecular weight standards is required to establish the actual exclusion volume and included volume for the samples, because their size and shape are affected by the method conditions, which can include sample/standard diluent and mobile phase.
For additional information, see How do I calculate the empty column volume of a column?
id88312, eluent, UPBINARY