How do I calculate the empty column volume of a column? - WKB48971

OBJECTIVE or GOAL

Determine the volume of an empty column.

ENVIRONMENT

• Column
• calculate
• empty volume
• HPLC
• UPLC
• ACQUITY
• HSS
• Symmetry
• BEH
• OST
• APC
• XBridge

PROCEDURE

1. Use Volume = pi x radius² x Length
2. pi = 3.14
3. r^₂ and Length should be converted to centimeters
4. Diameter of column divided by 2 = radius
5. radius x radius = r²
6. 3.14 x  r²  x L = Volume in cm³
7. cm³ = 1 mL

ADDITIONAL INFORMATION

The empty column volume of 4.6 mm x 150 mm is 2.5 mL.

pi = 3.14

4.6 mm  becomes 0.46 cm and 150 mm becomes 15 cm.

0.46 cm divided by 2 =  0.23 cm radius

0.23 cm x 0.23 cm = 0.0529 cm²

3.14 x 0.0529 cm² x 15.0 cm = 2.49cm³

Also see --> How do I determine column void volume?