# How do I calculate the empty column volume of a column? - WKB48971

Article number: 48971

## OBJECTIVE or GOAL

Determine the volume of an empty column.

## ENVIRONMENT

- Column
- HPLC
- UPLC
- ACQUITY
- HSS
- Symmetry
- BEH
- OST

## PROCEDURE

- Use Volume = pi x radius
^{2}x Length - pi = 3.14
- r
^{2}and Length should be converted to centimeters - Diameter of column divided by 2 = radius
- radius x radius = r
^{2} - 3.14 x r
^{2}x L = Volume in cm^{3} - cm
^{3}= 1 mL

## ADDITIONAL INFORMATION

The empty column volume of 4.6 mm x 150 mm is 2.5 mL.

pi = 3.14

4.6 mm becomes 0.46 cm and 150 mm becomes 15 cm.

0.46 cm divided by 2 = 0.23 cm radius

0.23 cm x 0.23 cm = 0.0529 cm^{2}

3.14 x 0.0529 cm^{2} x 15.0 cm = 2.49cm^{3}

Also see --> How do I determine column void volume?