What is the suggested injection volume size for an HPLC column? - WKB48961
Article number: 48961
ENVIRONMENT
- column
- HPLC
- Symmetry
- Symmetry300
- XBridge
- injection
- volume
ANSWER
The suggested injection volume size range is 1%-10% of the total column volume of the HPLC column.
ADDITIONAL INFORMATION
To minimize peak shape issues related to volume overload or strong solvent effect, the injection volume size should be closer to 1%-3% of total column volume. Ultimately, the response of the particular sample in the particular detector may also dictate the injection volume used to keep the response in the linear range.
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