How can I minimize secondary interactions (nonspecific binding) on a BEH SEC column? - WKB64185
Article number: 64185
ENVIRONMENT
- ACQUITY UPLC BEH SEC columns
- Size exclusion chromatography (SEC)
- XBridge BEH SEC columns
ANSWER
High-molarity (up to ~350mM) salt buffers are often used.
In some cases, a small amount of organic (<20%) in an aqueous solution is used.
ADDITIONAL INFORMATION
For additional information, see Size-Exclusion Chromatography (SEC) Optimization Guide and What are some suggestions for doing method development of hydrophobic proteins using BEH SEC columns?
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