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How can I minimize secondary interactions (nonspecific binding) on a BEH SEC Column? - WKB64185

Article number: 64185

ENVIRONMENT

  • ACQUITY UPLC BEH SEC Columns
  • XBridge BEH SEC Columns
  • Size Exclusion Chromatography
  • SEC
  • Column Use
  • Nonspecific Binding
  • Secondary Interactions

ANSWER

Use of high molarity salt buffers (up to ~350mM) is often used. 

In some cases a small amount of organic (<20%) in an aqueous solution is used.

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