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Will the AccQ-Tag Ultra Derivatization Reagent or the AccQ-Fluor Reagent react with any cell culture media components when analyzing the amino acid content of cell culture? - WKB64205

Article number: 64205

ENVIRONMENT

  • AccQ-Tag Ultra
  • AccQ-Tag 
  • AccQ-Fluor
  • Amino Acid Analysis
  • Cell Culture
  • derivatize

ANSWER

These reagents react with:

1.  Any molecule that contains a primary or secondary amine.

2. Water,  to form AMQ (6-aminoquinolone).  This is seen as the "AMQ" peak in AccQ.Tag Ultra and AccQ.Tag.

3. AMQ to form a dimer of AMQ which is bis-aminoquinonline urea.  This is the "derivatization peak" seen in AccQ.Tag Ultra Method.

4. Molecules which contain alcohol groups, at a slower rate then primary and secondary amines.

 

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