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Waters

What are some suggestions for doing method development for hydrophobic proteins using BEH SEC columns? - WKB72310

Article number: 72310

ENVIRONMENT

  • BEH SEC columns
  • Size-exclusion chromatography
  • ACQUITY
  • XBridge
  • Hydrophobic proteins

ANSWER

Consider using a lower-concentration salt mobile phase. High salt may expose more hydrophobic regions on the protein and enhance further secondary interactions.  

In addition to lowering the ionic strength of the salts, try an alternative salt, such as perchlorate- and lithium-based salts, on the Hofmeister series.

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