What are some suggestions for doing method development for hydrophobic proteins using BEH SEC columns? - WKB72310
Article number: 72310
ENVIRONMENT
- BEH SEC columns
- Size-exclusion chromatography
- ACQUITY
- XBridge
- Hydrophobic proteins
ANSWER
Consider using a lower-concentration salt mobile phase. High salt may expose more hydrophobic regions on the protein and enhance further secondary interactions.
In addition to lowering the ionic strength of the salts, try an alternative salt, such as perchlorate- and lithium-based salts, on the Hofmeister series.
ADDITIONAL INFORMATION
For additional information, see the Size-Exclusion Chromatography (SEC) Optimization Guide and How do I measure non-specific binding in an LC system for impact on peak shape in SEC of proteins?
id72310, eluent, UPBINARY