Skip to main content
Waters

What is a good column choice for retention of organic acids? - WKB61121

Article number: 61121

ENVIRONMENT

  • Organic acid analysis
  • ACQUITY UPLC HSS T3
  • ACQUITY UPLC BEH Amide
  • XSelect HSS T3
  • XBridge BEH Amide

ANSWER

For LC with UV or PDA detection using a phosphate buffer:  ACQUITY UPLC HSS T3  or XSelect HSS T3 (reverse-phase method)

For LC with MS-compatible mobile phases: ACQUITY UPLC BEH Amide or XBridge BEH Amide (HILIC method)

ADDITIONAL INFORMATION

It might also be possible to lower pH in order to make some organic acids neutral, which may make them more hydrophobic and better retained by reversed phase.

HSS T3 columns are more modern versions of the Atlantis dC18. The following application notebooks show separation of organic acids on Atlantis dC18:

Atlantis dC18 & HILIC Silica Applications Notebook

ACQUITY UPLC BEH Amide Applications Notebook

XBridge Amide Columns Applications Notebook

Not able to find a solution? Click here to request help.