What is a good column choice for retention of organic acids? - WKB61121
Article number: 61121
ENVIRONMENT
- Organic Acid Analysis
- ACQUITY UPLC HSS T3
- ACQUITY UPLC BEH Amide
- XSelect HSS T3
- XBridge BEH Amide
- Atlantis PREMIER BEH C18 AX
ANSWER
Reversed Phase:
- ACQUITY Premier HSS T3
- ACQUITY UPLC HSS T3
- XSelect Premier HSS T3
- XSelect HSS T3
- Atlantis T3
- Atlantis dC18
- CORTECS Premier T3
- CORTECS T3
Reversed-Phase Mixed mode:
- Atlantis PREMIER BEH C18 AX (reversed-phase mixed-mode method)
HILIC:
- ACQUITY Premier BEH Z-HILIC
- ACQIUTY Premier BEH Amide
- ACQUITY UPLC BEH Amide
- XBridge Premier BEH Amide
- XBridge BEH Amide
ADDITIONAL INFORMATION
It might also be possible to lower pH in order to make some organic acids neutral, which may make them more hydrophobic and better retained by reversed phase.
HSS T3 columns are more modern versions of the Atlantis dC18. The following application notebooks show separation of organic acids on Atlantis dC18:
Atlantis dC18 & HILIC Silica Applications Notebook
ACQUITY UPLC BEH Amide Applications Notebook
XBridge Amide Columns Applications Notebook
Atlantis and Atlantis PREMIER Columns Webpage
Related application note:
Separation of Organic Acids with Mixed-Mode LC Column and Mass Detector
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