What were the method conditions used for the application note "Separation of Organic Acids with Mixed-Mode LC Column and Mass Detector" (720006744)? - WKB194533

ENVIRONMENT

• Organic acid separation
• Method conditions
• ACQUITY UPLC H-Class
• PDA
• QDa

ANSWER

Instrument: ACQUITY UPLC H-Class with PDA
Column: Atlantis PREMIER BEH C18 AX, 1.7 µm, 2.1 x 100 mm
Column temp.: 30 °C
Flow rate: 0.35 mL/min
Mobile phase: (A) 50 mM ammonium formate and 0.9% formic acid
in water (pH=2.9); (B) 0.9% formic acid in acetonitrile;
(C) 0.9% formic acid in water
Gradient:
Time
(min) %A %B   %C   Curve
0.0      0    0   100      6 
1.4      0    0   100      6 
1.5    60    0     40      6 
5.0    60   40      0      6
7.0    60   40      0      6 
7.1      0    0   100      6 
8.0      0    0   100      6
Mass detector: ACQUITY QDa
Ionization mode: Negative
Capillary voltage: 0.8 kV
Cone voltage: 5 V
Probe temp.: 600 °C
Data management: Empower 3 CDS

ADDITIONAL INFORMATION

Separation of Organic Acids with Mixed-Mode LC Column and Mass Detector 

Atlantis PREMIER Columns Infographic

Atlantis Premier BEH C18 AX Columns Care and Use Manua

A New Mixed-Mode Reversed-Phase/Anion-Exchange Stationary Phase Based on Hybrid Particles