What were the method conditions used for the application note "Separation of Organic Acids with Mixed-Mode LC Column and Mass Detector" (720006744)? - WKB194533
Article number: 194533
ENVIRONMENT
- Organic acid separation
- Method conditions
- ACQUITY UPLC H-Class
- PDA
- QDa
ANSWER
Instrument: ACQUITY UPLC H-Class with PDA
Column: Atlantis PREMIER BEH C18 AX, 1.7 µm, 2.1 x 100 mm
Column temp.: 30 °C
Flow rate: 0.35 mL/min
Mobile phase: (A) 50 mM ammonium formate and 0.9% formic acid
in water (pH=2.9); (B) 0.9% formic acid in acetonitrile;
(C) 0.9% formic acid in water
Gradient:
Time
(min) %A %B %C Curve
0.0 0 0 100 6
1.4 0 0 100 6
1.5 60 0 40 6
5.0 60 40 0 6
7.0 60 40 0 6
7.1 0 0 100 6
8.0 0 0 100 6
Mass detector: ACQUITY QDa
Ionization mode: Negative
Capillary voltage: 0.8 kV
Cone voltage: 5 V
Probe temp.: 600 °C
Data management: Empower 3 CDS