What is the startup procedure for a BEH HILIC column? - WKB61214

ENVIRONMENT

• column
• HILIC
• BEH
• ACQUITY
• XBridge
• startup procedure
• care and use
• instructions

ANSWER

Before you use a new BEH HILIC column, you must flush it with 50/50 Acetonitrile:Water with 10mM of the buffer used in the method for 50 column volumes.

After the 50/50 Acetonitrile:Water with 10mM final buffer step, equilibrate the column for 20 column volumes in the initial conditions of a gradient or the isocratic mobile phase.

Note: If you are using a BEH HILIC column with low concentrations of mobile phase additives, you may need 100- 200 column volumes for complete equilibration. In addition, mobile phases that contain formate may also require a longer initial column equilibration.   

ADDITIONAL INFORMATION

For additional information, see ACQUITY BEH Getting started with HILIC, page 10 of ACQUITY UPLC BEH Columns Care and Use Manual