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Waters

Can I use the ACQUITY UPLC Glycan BEH Amide column to separate and quantitate 2-AB labeled glycans? - WKB53607

Article number: 53607

ENVIRONMENT

  • Glycan analysis
  • GlycoWorks
  • ACQUITY UPLC columns

ANSWER

Yes

ADDITIONAL INFORMATION

Yes, in addition to being able to separate RapiFluor-MS labeled released N-glycans, the ACQUITY UPLC Glycan BEH Amide column can be used to separate 2-AB labeled released N-glycans as well.

There is an example on page 7 of the document linked below, where Waters shows the use of an ACQUITY UPLC BEH Amide 130A,1.7µm, 2.1mm x 150mm column for the separation of both 2-AB and RapiFluor-MS labeled released N-glycans:

Robustness of RapiFluor-MS N-Glycan Sample Preparations and Glycan BEH Amide HILIC Chromatographic Separations

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