What can cause poor retention of an analyte on a column? - WKB51705
Article number: 51705
ENVIRONMENT
- Column
- Analyte retention
- C18
- Amide
- reverse phase
- HILIC
ANSWER
Poor or no retention of an analyte can be caused by inadequate column equilibration or re-equilibration times.
ADDITIONAL INFORMATION
Column retention can also be affected by incorrect storage solutions.
For additional information, see the column care and use manual for the column brand in use.
See also What is meant by "capacity factor" in chromatography? and How long should I equilibrate my column for at startup?
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