What is the column cleaning procedure to reduce sodium adducts in oligonucleotide LC/MS analysis? - WKB51754
Article number: 51754
ENVIRONMENT
- Column cleaning
- OST
- Oligonucleotide analysis
- ACQUITY
- XBridge
ANSWER
To reduce the absorbtion of alkali metal ions to the column surface, the recommended cleaning procedure is to incorporate at the end of the gradient a low pH procedure using 50/50 Methanol/0.1% formic acid for 1 column volume.
ADDITIONAL INFORMATION
For additional information, see The Impact of Adducts and Strategies to Control Them in IP-RPLC Based Oligonucleotide Analyses.
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