For how long should I equilibrate my column at startup? - WKB51703
Article number: 51703
ENVIRONMENT
- Column
- Equilibration
- equilibrate
- care and use
- column volume
ANSWER
The recommended equilibration is 20 column volumes.
ADDITIONAL INFORMATION
If using low ionic strength buffers, columns may need 50-200 column volumes to equilibrate for the first time.
For additional information, see the care and use manual for your column brand.
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