What is the difference between XBridge Premier Protein SEC 250A, XBridge BEH SEC 200A, and BioResolve SEC mAb columns? - WKB252444

ENVIRONMENT

• Column comparison
• ACQUITY
• XBridge
• Bridged ethylene hybrid (BEH)
• Size exclusion chromatography (SEC)
• High Performance Surfaces (HPS)
• Polyethylene oxide (PEO)
• MaxPeak Premier

ANSWER

XBridge Premier Protein SEC 250A columns:

• 250A pore size
• PEO-bonded BEH particles (prevent protein adsorption to the particle)
• 2.5-µm particle size
• MaxPeak High Performance Surfaces hardware (prevents protein adsorption to the stainless steel frits or column body)
• First choice for best performance

XBridge BEH SEC 200A columns:

• 200A pore size
• Diol-bonded BEH particles
• 2.5-µm and 3.5-µm particle sizes
• Traditional stainless steel hardware
• Require conditioning injections in order to achieve best recovery of proteins

BioResolve SEC mAb columns:

• 200A pore size
• Diol-bonded BEH particles
• 2.5-µm particle size only
• Traditional stainless steel hardware
• Should not require conditioning injections in most cases

ADDITIONAL INFORMATION

MaxPeak Premier Protein SEC Columns Infographic

BEH SEC Column Technology – Infographic

BioResolve SEC mAb Consumables- Infographic