Can I use a column packed with XBridge BEH 130A C18 for purifying a 30-kDa protein? - WKB241287
Article number: 241287
ENVIRONMENT
- Column care and use
- XBridge BEH 130A C18
- Protein purification
ANSWER
No, this is not recommended without experiments to optimize conditions and minimize protein carryover.
ADDITIONAL INFORMATION
Although it is possible to analyze larger peptides and proteins on this packing material, the best resolution for biomolecules is found between 1 kDa and 15 kDa.
For additional information, see When would I use a 300-angstrom pore versus a 130-angstrom pore for reverse-phase separation of biomolecules?
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