- Reverse-phase biomolecule separation
Small hydrophilic biomolecules are usually best separated using a 130-angstrom pore in reverse phase. A 300-angstrom pore is better suited for biomolecules larger than approximately 5 kilodaltons.
If the biomolecule is large and hydrophobic, as is the case with many proteins, consider using a C4 stationary phase as opposed to a C18.
For additional information, see Effect of Pore Size in UPLC Peptide Mapping.