When would I use a 300-angstrom pore versus a 130-angstrom pore for reverse-phase separation of biomolecules? - WKB57015
Article number: 57015
ENVIRONMENT
- Reverse-phase biomolecule separation
- Columns
- 300A
- 130A
- pore size
- reverse phase
- C18
- C4
- biomolecules
- peptide
- protein
ANSWER
Small hydrophilic biomolecules are usually best separated using a 130-angstrom pore in reverse phase. A 300-angstrom pore is better suited for biomolecules larger than approximately 5 kilodaltons.
ADDITIONAL INFORMATION
If the biomolecule is large and hydrophobic, as is the case with many proteins, consider using a C4 stationary phase as opposed to a C18.
For additional information, see Effect of Pore Size in UPLC Peptide Mapping.
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