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When would I use a 300-angstrom pore versus a 130-angstrom pore for reverse-phase separation of biomolecules? - WKB57015

Article number: 57015

ENVIRONMENT

  • Reverse-phase biomolecule separation
  • Columns

ANSWER

Small hydrophilic biomolecules are usually best separated using a 130-angstrom pore in reverse phase. A 300-angstrom pore is better suited for biomolecules larger than approximately 5 kilodaltons.

ADDITIONAL INFORMATION

If the biomolecule is large and hydrophobic, as is the case with many proteins, consider using a C4 stationary phase as opposed to a C18.

For additional information, see Effect of Pore Size in UPLC Peptide Mapping.

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