What is the void volume of my XBridge BEH450 SEC column? - WKB209538
Article number: 209538
ENVIRONMENT
- XBridge BEH450 SEC
- Column void volume
ANSWER
The void volume in SEC is the exclusion volume of the column.
Vo = exclusion volume. Use approximately 0.4 x empty SEC/GPC column volume.
Vt = total retention volume (words used in Empower GPC software parameter) [totally included volume]. Use approximately 0.8 x empty SEC/GPC column volume.
ADDITIONAL INFORMATION
Calibration of actual molecular weight standards is required to establish the actual exclusion volume and included volume for the samples, because their size and shape are affected by the method conditions, which can include sample/standard diluent and mobile phase.
For additional information, see How do I calculate the empty column volume of a column?
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