What is the best SEC column to analyze AAV9 and its aggregates? - WKB123662

ENVIRONMENT

• Size exclusion chromatography (SEC) 
• AAV
• Serotype AAV9

ANSWER

GTxResolve Premier BEH SEC 450A 2.5um Columns

XBridge Protein BEH SEC, 450 Å, 3.5 μm Columnns

ADDITIONAL INFORMATION

GTxResolve SEC Columns Web Page

GTxResolve Premier BEH 450 SEC Care and Use Manual

XBridge Protein BEH SEC Columns & Standards Care & Use Manual (lit code 720005206en)

Application notes:

Improved Size-Exclusion Chromatography of Adeno-Associated Virus (AAV) with GTxResolve Premier BEH SEC 450Å 2.5µm Columns   (Lit code 720007961)

Size-Exclusion Chromatography Analysis of Adeno-Associated Virus (AAV) Preparations Using a 450 Å Diol-Bonded BEH Column and Fluorescence Detection

Heightened Characterization of AAVs by SEC-MALS with GTxResolve Premier BEH SEC 450 Å 2.5 µm Columns   (lit code 720007969)

Note: No single mobile phase works for all different serotypes; optimal SEC mobile composition should be evaluated depending on what serotype of AAV is being analyzed.

Also see

 What are some scouting conditions to explore for SEC separation of 20-nm AAVs?