What are some scouting conditions to explore for SEC separation of 20-nm AAVs? - WKB90086
Article number: 90086
ENVIRONMENT
- Size exclusion chromatography (SEC)
- Adeno-associated virus (AAV)
ANSWER
20-nm AAVs have been successfully separated from the dimer using a mobile phase containing phosphate buffer and 350mM KCl at pH 6.6 and a flow rate of 0.5 mL/min on column 186007643 (XBridge BEH450 SEC 3.5 µm, 7.8 x 300 mm).
ADDITIONAL INFORMATION
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