Why does the peak shape decline as I inject higher volumes on a column? - WKB118871
Article number: 118871
ENVIRONMENT
- Peak shape
- Volume overload
- Strong solvent effect
ANSWER
The peak shape can be affected by volume overload and strong solvent effects.
ADDITIONAL INFORMATION
For additional information, see What is the suggested injection volume size to inject on an HPLC column? and Does Waters have an example showing mass overload versus volume overload on an LC column?
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