Is it possible to separate the mAb monomer and dimer (HMW, aggregate, LMW)? And what is the optimum pH and salt concentration for the separation conditions? - WKB118888

ENVIRONMENT

• BioResolve
• mAb monomer and dimer separation
• ACQUITY UPLC Protein BEH SEC

ANSWER

Separation is possible with the following columns:

ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 x 150 mm and BEH200 SEC Protein Standard Mix
Part number: 176003904

ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 4.6 mm X 150 mm, 10K --500K, 1 / pkg
Part number: 186005225

ACQUITY UPLC Protein BEH SEC Column 200Å, 1.7 µm, 4.6 x 300 mm and BEH200 SEC Protein Standard Mix
Part number: 176003905

ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pkg
Part number: 186005226

Find analytical data and optimal conditions in the Guide to Size-Exclusion Chromatography (SEC) of mAb Aggregates, Monomers, and Fragments (https://www.waters.com/waters/librar...9&locale=en_US).

BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 300 mm, 1/pk
Part number: 186009437
https://www.waters.com/waters/en_US/...&cid=135071423

ADDITIONAL INFORMATION