What is the optimal flow rate range for a CORTECS 2.7 µm, 4.6 x 150 mm column on an Alliance e2695 HPLC system? - WKB110442
Article number: 110442
ENVIRONMENT
- UltraPerformance Liquid Chromatography (UPLC)
- Ultra High Performance Liquid Chromatography (UHPLC)
- CORTECS columns
ANSWER
1.0 - 1.3 mL/min, depending on observed back pressure
ADDITIONAL INFORMATION
Waters has evaluated this flow rate range on an Alliance e2695 HPLC system with a TUV Detector.
The mobile phases used were 80:20 water/acetonitrile.
The column temperature was set to 30 °C.
Under these conditions, the total observed back pressures (column + system) were as follows:
At 1.0 mL/min = ~2,800 psi
At 1.3 mL/min = ~3,600 psi
NOTE: The total pressure may vary with the system used and how the system is configured.
CORTECS Solid Core Particle Columns Brochure
id110442, eluent