BioResolve SEC mAb columns address challenge of separating monomer from fragments - WKB110376

SYMPTOMS

• Poor resolution between the monomer and the fragments in SEC analysis

ENVIRONMENT

• SEC analysis
• BioResolve SEC mAb columns
• UPLC
• UHPLC
• HPLC

CAUSE

The prevalence of the secondary interaction with the currently used BEH SEC column leads to poor resolution of the monomer and the fragments.

FIX or WORKAROUND

• The recently launched BioResolve SEC mAb columns can address this challenge. The columns are designed specifically to help generate reliable mAb aggregate, monomer, and fragment separation with accurate quantification.
• Refined manufacturing processes and QC testing of every packed column help ensure that every column performs out of the box for this challenging analysis. Each column is QC tested with a NIST-based mAb size variant standard to help ensure column-to-column consistency when used on UPLC, UHPLC, or HPLC.
• This is a preconditioned column.
• The column was developed to accommodate optional use of a cost-effective BioResolve mAb Guard to increase analytical column lifetime without degrading necessary mAb component resolution and quantitation.

ADDITIONAL INFORMATION