How do I convert a Waters Spherisorb Amino (NH2) normal-phase column to run a reverse-phase or HILIC method? - WKB102321

OBJECTIVE or GOAL

Switch solvents from normal phase to reverse phase or HILIC.

ENVIRONMENT

• NH2
• Solvent switching
• Hydrophilic interaction chromatography (HILIC)
• Solvent changeover

PROCEDURE

1. Flush with 10 column volumes of 100% isopropanol.

2. Flush with 10 column volumes of 100% Acetonitrile to reduce backpressure when moving into next step

3. Flush with miscible aqueous-organic mobile phase with no buffer for 50 column volumes. (Example: 50:50 water/acetonitrile)

4. Flush with initial mobile phase with buffer (if using buffer) for 20 column volumes.

ADDITIONAL INFORMATION

Waters Spherisorb columns ship in 96:4 heptane/isopropanol.

Also see: 

What solvents do Waters Spherisorb Amino (NH2) columns ship in? 

Can I use a Waters Spherisorb NH2 (amino) column to analyze carbohydrates?

Waters Spherisorb Columns Webpage

Waters Spherisorb Columns Brochure

Waters Spherisorb Columns Care & Use Manual