What solvents do Waters Spherisorb Amino (NH2) columns ship in? - WKB50722
Article number: 50722
ENVIRONMENT
- column
- Waters Spherisorb
- Spherisorb
- NH2
- solvent
- shipped in
- reverse phase
- water
- Acetonitrile
ANSWER
96:4 heptane:isopropanol
ADDITIONAL INFORMATION
If performing a reversed phase or HILIC method, the column must first be converted to the reversed phase of HILIC solvents, as follows:
1. Flush with 10 column volumes of 100% isopropanol.
2. Flush with miscible aqueous-organic mobile phase with no buffer for 10 column volumes. (Example: 50/50 water:acetonitrile)
3. Flush with initial mobile phase with buffer (if using buffer) for 10 column volumes.
See Waters Spherisorb™ Columns Care & Use Manual (WAT094178): https://help.waters.com/help/en/support/library-details.html?documentid=WAT094178
id50722, eluent