What solvents do Waters Spherisorb Amino (NH2) columns ship in? - WKB50722

ENVIRONMENT

• column
• Waters Spherisorb 
• Spherisorb
• NH2
• solvent
• shipped in
• reverse phase
• water
• Acetonitrile

ANSWER

96:4 heptane:isopropanol

ADDITIONAL INFORMATION

If performing a reversed phase or HILIC method, the column must first be converted to the reversed phase of HILIC solvents, as follows:

1. Flush with 10 column volumes of 100% isopropanol.

2. Flush with miscible aqueous-organic mobile phase with no buffer for 10 column volumes. (Example: 50/50 water:acetonitrile)

3. Flush with initial mobile phase with buffer (if using buffer) for 10 column volumes.

See Waters Spherisorb™ Columns Care & Use Manual (WAT094178): http://www.waters.com/waters/support.htm?lid=10077655&type=USRM