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What is the shelf life of buffers used in reverse-phase separations of oligonucleotides? - WKB50725

Article number: 50725

ENVIRONMENT

  • Oligonucleotides
  • Reverse-phase separation
  • Ion pair buffers
  • HFIP/TEA
  • TEAA
  • OST
  • C18

ANSWER

The shelf life of ion pair buffers after preparing for retention of oligonucleotides is five days or less.

ADDITIONAL INFORMATION

Retention and selectivity of oligonucleotides is impacted by the concentration and hydrophobicity of the amine used in the buffer. The volatility of the buffer will change the concentration of the amine ions in solution. In addition, trace metal adducts will leach from mobile phase containers. The adducts will interact with the backbone of the oligonucleotides and impact retention as well.

For additional information, see The Impact of Adducts and Strategies to Control Them in IP-RPLC Based Oligonucleotide Analyses.

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