Can I use a Waters Spherisorb NH2 (amino) column to analyze carbohydrates? - WKB102319
Article number: 102319
ENVIRONMENT
- High Performance Liquid Chromatography (HPLC)
- Column
- Carbohydrates
- oligosaccharides
- sugar
- Amino (NH2)
- Hydrophilic Interaction Chromatography (HILIC)
ANSWER
Yes, provided the solvent is switched to a HILIC solvent system.
ADDITIONAL INFORMATION
An example of HILIC solvents used to retain carbohydrates on this column is 80:20 acetonitrile:water.
For additional information on how to switch solvents on Waters Spherisorb NH2 column for use in reversed-phase or HILIC separations, refer to What solvents do Waters Spherisorb Amino (NH2) columns ship in?
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