REM Method Editor

To acquire sample data in REM mode

1. Open MassLynx, and then select the Instrument tab.
2. Click MS Method.

3. Select REM in the Analyser Mode drop-down menu.

4. Ensure that the Lock Mass to be used is appropriate for the REM mass range selected.

NOTE:    The precursor mass of leucine enkephalin (m/z 556.27658 / 554.26202) and can be used for the 200-600 m/z and 300-1000 m/z mass ranges whereas the following fragment ion of leucine enkephalin can be used for the 100-300 m/z mass range:

Positive ion (M+H)⁺ = C₁₁H₁₃N₂O₃ = 221.09207 (Neutral = C₁₁H₁₂N₂O₃)

Negative ion (M-H)^- = C₁₁H₁₄N₃O₃ = 236.10406 (Neutral = C₁₁H₁₅N₃O₃)

5. Click Save.
6. Select the appropriate ms method files for sample analysis.