2545 retention time shifts with analytical flow rate - WKB82031

SYMPTOMS

• Peak retention time shifts

ENVIRONMENT

• 2525/2545 Binary Gradient Module
• 2535/2545/2555 Quaternary Gradient Module

CAUSE

The accuracy of online solvent mixing at analytical flow rates (<1.0 mL/min) is reduced due to large pump head volumes.

FIX or WORKAROUND

Install the following mixer between the pump and the injector.

Mixer (700011657)

ADDITIONAL INFORMATION

The mixer can remain in the flow path for preparative and analytical methods.