What is the definition of stroke volume in Alliance 2695 context? - WKB299408

ENVIRONMENT

• Alliance 2695

ANSWER

Stroke volume refers to the volume of solvent delivered with each piston stroke in the context of the Alliance HPLC System and the 2695 Separations Module. The separations module's discrete, programmable stroke volumes provide optimal flow delivery and solvent blending. The default (preprogrammed) stroke volume is typically 130 µL. However, this can be overridden to select a preferred stroke volume of 25 µL, 50 µL, or 100 µL for specific applications. A lower stroke volume can be more effective with solvents that require additional mixing, such as trifluoroacetic acid (TFA) gradients. This additional mixing occurs without added delay volume. The stroke volume can be set in method parameters in Empower or on the front panel of the Alliance HPLC system. For example, for a flow range of 0.050–0.530 mL/min, a stroke volume of 25 µL is recommended. For a flow range of 0.531–1.230 mL/min, a stroke volume of 50 µL is recommended. For a flow range of 1.231–3.030 mL/min, a stroke volume of 100 µL is recommended. And for a flow range of 3.031–5.00 mL/min, a stroke volume of 130 µL is recommended 

ADDITIONAL INFORMATION