With a Tof MS, how do you quantify with TIC? - WKB94908

ENVIRONMENT

• Xevo G2-XS QTof
• SYNAPT G2-Si

ANSWER

Quantify with the total value of 50-1000 Da spectra.

When performing the Tof MS quantitation, use the chromatogram extracted at m/z xxx for the target compound ion.

For a chromatogram that combines all spectra in a wide range:
Unless the following conditions are met, it is not generally possible to obtain good linearity, so it is not recommended.

• Background ions (ions other than the target compound) are always present with a constant intensity
• The types of background ions are always constant
• No ions are intense enough to saturate in the background ions

ADDITIONAL INFORMATION