How to run Detector Setup with GFP - WKB85964

OBJECTIVE or GOAL

• Perform Detector Setup with GFP

ENVIRONMENT

• Xevo G2-XS

PROCEDURE

<Create a new reference file>

1. Select Reference File Editor from the Configure menu.
2. Select Detector Setup (Leucine Enkephalin), right click and select Copy.
3. There is a line called “Detector Setup (Leucine Enkephalin) -Copy”. Right click on this line and select Edit.
4. Change the Reference Compound name to “Detector Setup (GFP)”.

• At this time, change the “Leucine Enkephalin” characters to “GFP” and delete “-Copy”.
• Do not edit other characters, such as “Detector Setup” or “()”.

5. Select Positive for Polarity and MS for Mode.

• Register 785.8426 and 1570.6774 in the Positive field.
• Set 785.8421 to 100% and 1570.6774 to 55%.

Create a Detector Setup (GFP) for Negative in the same way.

6. Select the Detector Setup (GFP) created above, right-click and select Copy.
7. There will be a line called “Detector Setup (GFP)-Copy”. Right click on this line and select Edit.
8. Select Negative for Polarity and MS for Mode.

• Register 783.8270 and 1568.6617 in the Negative field.
• Set 783.8275 to 100% and 1568.6617 to 55%.

 

<Running the Detector Setup>

1. Check “Detector Setup” in Intellistart and click Start button.
2. Change the settings on the Detector Setup window as shown below and perform Detector Setup.

• In the Reference Compound field, select Detector Setup(GFP).
• Confirm that 785.8421 is displayed in the Mass(Da) column in Positive mode.
• To perform Detector Setup only in Positive mode, select “Do not setup Detector for Negative polarity” for Negative mode.

ADDITIONAL INFORMATION