How to calibrate the LCT Premier XE using the calibration wizard - WKB32438

Article number: 32438

OBJECTIVE or GOAL

To successfully calibrate the LCT Premier or LCT Premier XE

Infuse sodium formate solution into the analyte probe and ensure that a spectrum of sodium formate is observed on the MS Tune page.
Run the Calibration Wizard, selecting a mass range of 50 to 1000 Da, and the reference file NaFormatePosES.ref.
Ensure that a calibration curve with a RMS residual of less than <5e-04 amu (or less than 3 ppm) is produced.
4. If required, click Edit > Calibration Parameters and vary the intensity threshold to remove any small peaks from consideration by the software.
NOTE: If required, the polynomial value can be adjusted to achieve a better mathematical fit for the calibration curve.
Outlier peaks that are affecting the calibration fit (such as those of insufficient intensity) can be removed from the calibration.
6. If required, left-click and drag to zoom in on the peak to be removed, then right-click on the peak in the middle trace, then right-click on the peak in the top trace to remove it from the calibration calculation.
NOTE: The removal of too many peaks results in a suspect calibration calculation.
Click Finished > Accept Calibration… once optimization is complete to accept the calibration.
Click Finish to complete the Wizard.

id32438, LCTPREMIER, LCTPREMXE