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Contamination ions observed on ACQUITY SYNAPT G2 system - WKB24900

Article number: 24900

SYMPTOMS

  • More than five contaminant ions observed between masses 350 and 1000

ENVIRONMENT

  • ACQUITY
  • SYNAPT G2
  • LCMS

CAUSE

Contamination from prior samples

FIX or WORKAROUND

  1. Remove the column and replace it with a union.
  2. Put the A and B lines from the ACQUITY into the alcohol mix, which is 25/25/25/25 water/isopropyl alcohol/methanol/acetonitrile and 0.2% formic acid (can go up to 1% formic acid).
  3. Flush the system by priming for five minutes, and then pump for at least two hours (preferably overnight) at a flow rate that gives at least 6000-psi pressure.
  4. Prime and flush with fresh LC/MS-grade or Milli-Q water. 
  5. Put on fresh LC/MS-grade solvents for the LC/MS analysis. If the solvent bottles were not cleaned recently, use the alcohol mix to clean out the bottles, and then rinse them thoroughly with fresh solvent. 
  6. Attach a new column.
  7. Prime and flush out the water using the fresh solvents.
  8. Run the LC/MS analysis again. 

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