Fluidics system is not infusing sample - WKB125181

SYMPTOMS

• No signal when infusing sample
• No signal when infusing setup solution
• Spray is restored when HT diverter valve is switched to LC state (LC must be flowing)

ENVIRONMENT

• MassLynx
• Xevo TQ-S
• Xevo G2-XS QTof
• Xevo TQ
• Synapt G2
• Synapt G2-Si

CAUSE

The bottle reservoir lines are dry or air locked.

FIX or WORKAROUND

1. Place the fluidics wash line (orange PEEK) in 100% IPA (possibly MS-grade), set fluidics to "Wash", and purge the system several times.
2. Fill a HPLC vial with 100% IPA (possibly MS-grade) and attach it to reservoir A, set fluidics to "Reservoir A", purge the system, and confirm that solvent level drops. If this does not help, backflush the reservoir line with the LC flow, connecting with a union the LC tubing and the selected reservoir line tubing.
3. Repeat for the remaining reservoir B and C lines if necessary.

ADDITIONAL INFORMATION

Alternatively to the LC flow, you can use a syringe (equipped with a blunt needle and a nut and ferrule) for flushing the line and removing the clog or air bubbles.