Fluidics system is not infusing sample - WKB125181

SYMPTOMS

• No signal when infusing sample
• No signal when infusing setup solution
• Spray is restored when HT diverter valve is switched to LC state (LC must be flowing)

ENVIRONMENT

• MassLynx
• Xevo TQ-S
• Xevo G2-XS QTof
• Xevo TQ
• Synapt G2
• Synapt G2-Si

CAUSE

The bottle reservoir lines are dry or air locked.

FIX or WORKAROUND

1. Place the fluidics wash line (orange PEEK) in 100% IPA (possibly MS-grade), set fluidics to "Wash", and purge the system several times. Fill a HPLC vial with 100% IPA (possibly MS-grade) and attach it to reservoir A, set fluidics to "Reservoir A", purge the system, and confirm that solvent level drops. Repeat for the remaining reservoir B and C lines if necessary.

ADDITIONAL INFORMATION

If the fix listed above does not help, backflush the reservoir line with the LC flow, connecting with a union the LC tubing and the selected reservoir line tubing.

Alternatively to the LC flow, you can use a syringe (equipped with a blunt needle and a nut and ferrule) for flushing the line and removing the clog or air bubbles.