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Peak splitting with Alliance HPLC System and 2489 UV/Vis Detector - WKB94652

Article number: 94652

SYMPTOMS

  • Peak splitting for one sample but not the rest

ENVIRONMENT

  • Alliance HPLC System
  • 2489 UV/Vis Detector
  • ACQUITY UPLC H-Class

CAUSE

Something is building up at the front end of the column.

FIX or WORKAROUND

  1. Clean the column by back-flushing, or replace the column.

ADDITIONAL INFORMATION

 

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