- Negative peaks on 2414 Refractive Index Detector
- Also see negative peaks in blank injections which is mobile phase
- 2414 Refractive Index Detector
- Empower 3
FIX or WORKAROUND
- Use a different water source
Troubleshooting tip: Double or triple the injection volume. If the peak area increases, then the contamination is in the mobile phase. If the peak area stays the same, then the contamination is somewhere in the system.