With 2414 RID, poor reproducibility after switching solvents - WKB83898

SYMPTOMS

• High area %RSD
• High peak height %RSD
• Noisy baseline

ENVIRONMENT

•  2414 Refractive Index Detector
• ACQUITY UPLC
• Alliance HPLC
• HPLC modules

CAUSE

Not equilibrating long enough after switching solvents when using the 2414 Refractive Index Detector

FIX or WORKAROUND

1. Purge the detector until the baseline is stable.
2. Equilibrate the detector at initial conditions for at least 30 minutes.

ADDITIONAL INFORMATION

Waters 2414 Refractive Index Detector Operator’s Guide