With 2414 RID, poor reproducibility after switching solvents - WKB83898
Article number: 83898
SYMPTOMS
- High area %RSD
- High peak height %RSD
- Noisy baseline
ENVIRONMENT
- 2414 Refractive Index Detector
- ACQUITY UPLC
- Alliance HPLC
- HPLC modules
CAUSE
Not equilibrating long enough after switching solvents when using the 2414 Refractive Index Detector
FIX or WORKAROUND
- Purge the detector until the baseline is stable.
- Equilibrate the detector at initial conditions for at least 30 minutes.